Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Donnan equilibrium in the tectorial membrane.

K P Steel

    Hearing Research
    |November 1, 1983
    PubMed
    Summary

    The tectorial membrane exhibits a negative potential, influenced by pH and ionic strength. This suggests it acts as a distinct phase, challenging assumptions of in vivo electrical transparency.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genetic deafness of central origin.

    Brain research·2013
    Same author

    Pitpnm1 is expressed in hair cells during development but is not required for hearing.

    Neuroscience·2013
    Same author

    Presence of interstereocilial links in waltzer mutants suggests Cdh23 is not essential for tip link formation.

    Neuroscience·2008
    Same author

    The roles of unconventional myosins in hearing and deafness.

    Essays in biochemistry·2002
    Same author

    Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations.

    Nature neuroscience·2001
    Same author

    The Wheels mutation in the mouse causes vascular, hindbrain, and inner ear defects.

    Developmental biology·2001

    Area of Science:

    • Otoacoustic emissions and inner ear physiology
    • Biophysics of cochlear structures

    Background:

    • The tectorial membrane is a key component of the cochlea, involved in auditory transduction.
    • Its electrical and ionic properties in vivo are not fully understood.

    Purpose of the Study:

    • To investigate the basic electrical properties of the isolated tectorial membrane.
    • To determine the influence of bathing fluid composition on membrane potential.

    Main Methods:

    • Equilibrating isolated tectorial membrane samples in various bathing fluids.
    • Measuring potential difference using micropipette electrodes.

    Main Results:

    • A negative potential was consistently recorded within the tectorial membrane matrix.
    • The magnitude of this negative potential varied with the pH and ionic strength of the surrounding fluid.
    • These findings indicate a Donnan equilibrium is established.

    Conclusions:

    • The tectorial membrane functions as a distinct phase in vitro.
    • In vivo assumptions of electrical and ionic transparency may be inaccurate.
    • Further research is needed to understand its physiological role.

    Related Experiment Videos