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[Method of determining serum iron and iron-binding capacity without protein precipitation in very young children]
Insights
A modified bathophenanthroline method accurately diagnoses and monitors anemia treatment in young children by measuring iron levels. This reliable method ensures effective anemia management in pediatric care.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Pediatric Hematology
Context:
- Anemia diagnosis and treatment monitoring in early childhood require accurate iron level assessment.
- Existing methods for iron quantification may have limitations in pediatric applications.
- The bathophenanthroline method is a known technique for iron determination.
Purpose:
- To describe a modified bathophenanthroline method for quantifying iron in biological samples.
- To validate the modified method for diagnosing anemia and monitoring its treatment in young children.
- To establish normal iron and iron-binding capacity values in healthy children using the modified assay.
Summary:
- A modified bathophenanthroline method was developed and applied for anemia diagnostics and treatment control in young children.
- The method's accuracy was confirmed through iron concentration estimations in solutions and spiked blood serum, showing low standard deviations (0.5%-10%).
- Normal serum iron and iron-binding capacity values obtained in 30 healthy children aligned with existing literature data.
Impact:
- Provides a validated, reliable method for pediatric anemia management.
- Facilitates accurate diagnosis and effective monitoring of anemia treatment in early childhood.
- Contributes to improved health outcomes for children suffering from iron-related disorders.
Abstract:
A modification of bathophenanthroline method is described. The method was used for diagnostics of anemia and in order to control the treatment of anemia in children of early ages. The method was checked by estimation of known concentrations of iron in solutions as well as by addition of known amounts of iron to blood serum. Standard deviations ranged from +/- 1.04 to +/- 6.5 and variation coefficient--from 0.5% to 10%. Normal values of iron content and of iron-binding capacity of blood serum, estimated by means of the modified method in 30 healthy children of early ages, did not differ from the corresponding data published in the literature.