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[Thrombopoiesis, thrombocyte count and thrombocyte function before and following cell separation].
Klinische Wochenschrift
|October 3, 1983
Summary
Plateletpheresis, a cell separation technique, can yield 50% of circulating platelets. This study found that platelet removal stimulates thrombopoiesis, increasing platelet count and collagen-induced aggregation within 48 hours.
Area of Science:
- Hematology
- Cell Biology
- Biotechnology
Background:
- Plateletpheresis is a common procedure for obtaining platelets from healthy donors.
- The impact of platelet removal on thrombopoiesis, the process of platelet production, requires further investigation.
Purpose of the Study:
- To investigate the effects of cell separation on thrombopoiesis.
- To analyze morphometric changes in megakaryocytes and assess platelet function after platelet removal.
Main Methods:
- Bone marrow samples were collected from 12 donors up to 5 times.
- Megakaryocytes were analyzed using morphometric measures.
- Platelet count and functions (adhesion, aggregation, spreading, retraction) were determined before and after cell separation.
Main Results:
- Platelet removal stimulated thrombopoiesis, leading to an increased peripheral platelet count within 48 hours.
- Of the tested platelet functions, only aggregation induced by collagen showed a significant increase post-cell separation.
Conclusions:
- Cell separation, by stimulating thrombopoiesis, effectively increases platelet count.
- Enhanced collagen-induced platelet aggregation is a notable functional change following platelet removal.