Co-purification of coenzyme-dependent enzymes by affinity chromatography

Recent developments in general ligand affinity chromatography have greatly facilitated the purification of numerous coenzyme-dependent enzymes in practical scale. By immobilizing various derivatives of coenzymes (NAD+, NADP+, ATP and coenzyme A) on Sepharose, these general ligand affinity columns have the potential of enriching as many as 30% of the two thousand known enzymes as well as their isozymic forms, if they are applied in combination with the conventional procedures.