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The polypeptide of a human calicivirus
Archives of Virology
|January 1, 1983
Summary
Researchers identified a major structural protein in human calicivirus-like particles from gastroenteritis patients. This finding supports classifying these viruses within the Caliciviridae family.
Area of Science:
- Virology
- Gastroenterology
- Molecular Biology
Background:
- Acute gastroenteritis is a common human illness.
- Caliciviruses are known causes of gastroenteritis in animals.
- Human calicivirus-like particles have been observed in patients with gastroenteritis.
Purpose of the Study:
- To characterize the structural proteins of human calicivirus-like particles found in a patient with acute gastroenteritis.
- To determine if these particles belong to the Caliciviridae family.
Main Methods:
- Purification of viral particles from fecal samples.
- Radiolabeling of purified particles with Iodine-125 ([125I]).
- Analysis using SDS-PAGE and immunoprecipitation techniques.
Main Results:
- Viral particles resembling animal caliciviruses were isolated.
- A single major structural protein with a molecular mass of 62,000 daltons was identified.
- Immunoprecipitation confirmed this protein as the major structural component.
Conclusions:
- The identified human calicivirus-like particles possess characteristics consistent with the Caliciviridae family.
- These findings suggest a potential etiological link between these particles and acute gastroenteritis in humans.