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Effect of cycloheximide administration on bupivacaine-induced acute muscle degradation
Abstract:
We have examined the effect of cycloheximide on bupivacaine-induced, macrophage-mediated muscle degeneration in rats and obtained the following results. Direct intramuscular injection of bupivacaine into soleus muscle caused uniform muscle degeneration (Ishiura, S. et al. (1983) J. Biochem. 94, 311-314). The degeneration was most prominent 48 h after injection, with many infiltrating macrophages. Intraperitoneal administration of cycloheximide prevented the bupivacaine-induced macrophage invasion and decrease of structural proteins 48 h after injection. Cycloheximide inhibited the increase in lysosomal enzymes in bupivacaine-treated muscle. The peritoneal macrophages increased in number immediately after bupivacaine injection, while cycloheximide inhibited their accumulation. These results indicated that cycloheximide inhibited muscle degradation by preventing macrophage proliferation.
Insights
Cycloheximide prevents muscle degeneration caused by bupivacaine by inhibiting macrophage proliferation and invasion. This finding offers insights into managing drug-induced muscle damage.
Area of Science:
- Muscle physiology and pathology
- Immunology
- Pharmacology
Background:
- Bupivacaine injection causes muscle degeneration mediated by macrophages.
- Macrophage invasion and lysosomal enzyme increase are key features of this degeneration.
Purpose of the Study:
- To investigate the effect of cycloheximide on bupivacaine-induced muscle degeneration.
- To determine if cycloheximide can prevent macrophage proliferation and subsequent muscle damage.
Main Methods:
- Intramuscular injection of bupivacaine into rat soleus muscle.
- Intraperitoneal administration of cycloheximide.
- Assessment of muscle degeneration, macrophage infiltration, and lysosomal enzyme activity at 48 hours post-injection.
Main Results:
- Bupivacaine induced significant muscle degeneration and macrophage infiltration.
- Cycloheximide administration prevented bupivacaine-induced macrophage invasion and decrease in structural proteins.
- Cycloheximide inhibited the rise in lysosomal enzymes and peritoneal macrophage accumulation.
Conclusions:
- Cycloheximide effectively inhibits bupivacaine-induced muscle degradation.
- The mechanism involves preventing macrophage proliferation and subsequent tissue damage.