A simple method for testosterone measurement by high-performance liquid chromatography-UV spectrophotometer

Area of Science:

• Analytical Chemistry
• Biochemistry
• Pharmacology

Background:

• Accurate quantification of testosterone is crucial for endocrinological and pharmacological research.
• Existing methods for testosterone measurement may lack speed, simplicity, or reliability.
• Development of novel analytical techniques is needed for efficient biological sample analysis.

Purpose of the Study:

• To develop a simple, rapid, and reliable method for testosterone separation and quantitation.
• To establish a high-performance liquid chromatography-UV spectrophotometer technique for biological samples.
• To facilitate endocrinological pharmacology studies in animals and humans.

Main Methods:

• High-performance liquid chromatography (HPLC) with UV spectrophotometry was employed.
• Separation was achieved on a micro silica gel column using isocratic elution.
• The mobile phase consisted of hexane:chloroform:methanol (3:1:1).

Main Results:

• Testosterone and prednisolone (internal standard) were separated in under 3 minutes.
• The method accurately quantifies testosterone down to 5 ng in biological samples.
• The developed HPLC method demonstrates high efficiency and reliability.

Conclusions:

• A simple, rapid, and reliable HPLC-UV method for testosterone analysis in biological samples has been established.
• This technique enables accurate quantitation of low testosterone concentrations.
• The method offers a valuable new tool for endocrinological pharmacology research.