Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Changes in cell-surface sialic acid content during chick embryo development.

P Codogno, M Aubery

Mechanisms of Ageing and Development

|November 1, 1983

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Long-Lived Protein Degradation During Autophagy.

Methods in enzymology·2017

Same author

Molecular Mechanisms of Noncanonical Autophagy.

International review of cell and molecular biology·2017

Same author

Autophagy and regulation of cilia function and assembly.

Cell death and differentiation·2014

Same author

Beclin 1 and autophagy are required for the tumorigenicity of breast cancer stem-like/progenitor cells.

Oncogene·2012

Same author

Proton pump inhibition induces autophagy as a survival mechanism following oxidative stress in human melanoma cells.

Cell death & disease·2011

Same author

Human keratinocyte models: Assessment of cell adhesion and dermotoxicity using fluorescent probes.

Toxicology in vitro : an international journal published in association with BIBRA·2010

Same journal

Human sex-specific hormone effects on cerebrovascular health in males and females.

Mechanisms of ageing and development·2026

Same journal

HTZ-1/H2A.Z expression sustains transcriptional programs that regulate Caenorhabditis elegans lifespan.

Mechanisms of ageing and development·2026

Same journal

PCSK9 promotes aging-related cardiac calcification by inducing osteogenic differentiation of cardiac fibroblasts.

Mechanisms of ageing and development·2026

Same journal

Distinct post-infectious TLR2 immune remodeling in COVID-19-recovered centenarians.

Mechanisms of ageing and development·2026

Same journal

Senescence-associated tertiary lymphoid structures in Sjögren's disease model nishiura mice.

Mechanisms of ageing and development·2026

Same journal

Aging-driven reprogramming of CD34⁺ hematopoietic stem cells in leukemogenesis: Mechanisms and therapeutic implications.

Mechanisms of ageing and development·2026

See all related articles

Chick fibroblast cell-surface sialic acid increases with embryo development. However, this rise in sialic acid does not impact cell adhesion or detachment, suggesting it plays a minor role in these processes.

Area of Science:

Cell Biology
Developmental Biology
Biochemistry

Background:

Cell surface molecules, such as sialic acids, play crucial roles in cell-cell interactions and adhesion.
Embryonic development involves significant changes in cell behavior, including adhesion and detachment.

Purpose of the Study:

To investigate the changes in cell-surface sialic acid content during chick embryo fibroblast development.
To determine the relationship between cell-surface sialic acid levels and the adhesive properties of chick embryo fibroblasts.

Main Methods:

Quantification of cell-surface sialic acid released by trypsin from chick fibroblasts at different embryonic days (8 and 16).
Assessment of cell detachment and re-adhesion rates from substrate using trypsin treatment.
Enzymatic treatment with neuraminidase to evaluate the role of sialic acid in adhesion.

Related Experiment Videos

Main Results:

Cell-surface sialic acid content significantly increased from day 8 to day 16 of chick embryo development.
Younger (8-day) fibroblasts detached and re-adhered more rapidly than older (16-day) fibroblasts.
Neuraminidase treatment did not alter cell detachment or re-adhesion rates, indicating sialic acid's limited role.

Conclusions:

The increase in cell-surface sialic acid during chick embryo development is not the primary factor influencing cell adhesive capacity.
Cell-surface sialic acid appears to play a minimal role in the detachment and re-adhesion of embryonic fibroblasts.