Related Experiment Videos
Isolation of bovine polymorphonuclear leukocytes by density gradient centrifugation
Veterinary Immunology and Immunopathology
|December 1, 1983
Summary
Researchers developed a simple method to isolate over 95% pure bovine polymorphonuclear leukocytes (PMNs) using density gradient centrifugation. The isolated PMNs remained viable and functional for further research.
Area of Science:
- Veterinary Immunology
- Cell Biology
- Hematology
Background:
- Bovine polymorphonuclear leukocytes (PMNs) play a crucial role in the innate immune response.
- Efficient isolation of pure and functional bovine PMNs is essential for studying bovine immunity.
- Existing methods may lack efficiency or yield compromised cell viability.
Purpose of the Study:
- To develop and validate a simple, effective method for isolating highly enriched bovine PMNs.
- To ensure the viability and functionality of isolated PMNs for subsequent assays.
- To provide a reliable source of bovine PMNs for immunological research.
Main Methods:
- Isolation of leukocytes from bovine peripheral blood.
- Density gradient centrifugation using a Percoll medium with a specific gravity of 1.092.
- Assessment of cell viability and PMN functionality through migration inhibition and chemiluminescence assays.
Main Results:
- Achieved an isolation of greater than 95% pure bovine polymorphonuclear leukocytes (PMNs).
- Maintained high cell viability (greater than or equal to 95%) post-isolation.
- Demonstrated preserved functionality of isolated PMNs in key immunological assays.
Conclusions:
- Density gradient centrifugation offers a simple and effective method for obtaining high-purity bovine PMNs.
- The developed method yields viable and functional PMNs suitable for diverse functional studies.
- This technique facilitates research into bovine innate immunity and inflammatory responses.