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Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation
Analytical Biochemistry
|December 1, 1983
Abstract:
A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA--reversibly precipitated with the cationic detergent cetylpyridinium bromide--can be subjected to further enzymatic manipulations within minutes. This method is applied to the rapid separation of pBR322 plasmid from the chromosomal DNA.