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Immunochemical studies of the non-specific interactions of cyanogen bromide-activated macroporous agarose-based
Abstract:
This paper reports studies of the origin of the undesirable non-specific adsorption in immunoadsorption chromatography. The non-specificity of cyanogen bromide (CNBr)-activated macroporous agarose (Sepharose 4B)-based immunoadsorbents has been assessed from a comparative study of the following parameters: (1) The non-specific adsorption of protein (IgG) on unsubstituted cyanogen bromide-activated Sepharose 4B. (2) The non-specific adsorption of proteins (human serum IgG and sheep IgG) on cyanogen bromide-activated Sepharose 4B-sheep IgG immunoadsorbent. (3) The non-specific adsorption of proteins, immunoglobulin G(IgG) and human serum albumin (HSA) on cyanogen bromide-activated Sepharose 4B-sheep anti-human IgG immunoadsorbent and (4) the non-specific adsorption of peptides and amino acids on cyanogen-bromide activated Sepharose 4B-sheep anti-human IgG immunoadsorbent.