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Updated: Jul 25, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
Further studies on age-related unusual catecholamine structures in brain
Abstract:
The occurrence of large, intensely fluorescent (LIF) catecholamine-containing spots in the brains of mice and rats has been studied by fluorescence microscopy. LIF spots in C57BL/6J mice increased in number progressively with age. Apparent differences between sexes in the number of spots were not significant at any age. A greater number of LIF spots were present in older mice and rats of different strains indicating that LIF spot formation is neither strain nor species specific. Long-term feeding of antioxidants (ascorbic acid, alpha-tocopherol, and beta-mercaptoethylamine) to C57BL/6J mice did not alter significantly the number of LIF spots, suggesting that age-related LIF spots are formed by a mechanism other than endogenous free radical damage.
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