Analysis of phospholipids in human semen by high-performance liquid chromatography

A sensitive method for the separation of phospholipids by a high-performance liquid chromatographic procedure is described. The chromatographic separation was achieved on a 25-cm column packed with Bio-Sil HP-10 coupled with a pre-column packed with Si-100 Polyol. Phosphatidylinositol, phosphatidylglycerol, cardiolipin, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin, lysophosphatidylethanolamine and lysophosphatidylcholine were completely separated and quantitated. The eluted phospholipids were monitored at 203 nm. The method was shown to be applicable to the analysis of phospholipids from human semen.