Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Models for hydrogen exchange from folded proteins. II.

L M Ellis

    Biophysical Journal
    |July 1, 1978
    PubMed
    Summary

    This study models protein hydrogen exchange kinetics using two rate constant distributions, kcx and kn, for folded and unfolded states. The model successfully explains temperature-dependent exchange in soybean trypsin inhibitor across different pH levels.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Correction: Therapeutic targeting of Id2 reduces growth of human colorectal carcinoma in the murine liver.

    Oncogene·2020
    Same author

    Colorectal premalignancy is associated with consensus molecular subtypes 1 and 2.

    Annals of oncology : official journal of the European Society for Medical Oncology·2018
    Same author

    The requirement for freshly isolated human colorectal cancer (CRC) cells in isolating CRC stem cells.

    British journal of cancer·2014
    Same author

    Chemoresistant colorectal cancer cells and cancer stem cells mediate growth and survival of bystander cells.

    British journal of cancer·2011
    Same author

    Chronic exposure of colorectal cancer cells to bevacizumab promotes compensatory pathways that mediate tumour cell migration.

    British journal of cancer·2011
    Same author

    Intracrine vascular endothelial growth factor signaling in survival and chemoresistance of human colorectal cancer cells.

    Oncogene·2010

    Area of Science:

    • Biochemistry
    • Protein Dynamics
    • Chemical Kinetics

    Background:

    • Protein structure and dynamics are crucial for function.
    • Hydrogen exchange (HX) is a key technique for studying protein conformational dynamics.
    • Understanding exchange kinetics provides insights into protein folding and stability.

    Purpose of the Study:

    • To develop a kinetic model for protein hydrogen exchange.
    • To analyze exchange rates from folded and unfolded protein conformations.
    • To investigate the temperature dependence of hydrogen exchange in soybean trypsin inhibitor.

    Main Methods:

    • Modeling hydrogen exchange kinetics using continuous distributions of rate constants.
    • Analyzing exchange rates for folded (kcx) and unfolded (kn) conformations.
    • Experimental validation using soybean trypsin inhibitor at varying temperatures and pH.

    Main Results:

    • A model based on two rate constant distributions (kcx and kn) accurately describes protein hydrogen exchange.
    • The model accounts for the temperature dependence of exchange in soybean trypsin inhibitor at pH 3 and 6.5.
    • The distribution of rate constants for unfolded conformations (kn) provides significant physical insights.

    Conclusions:

    • The developed kinetic model offers a robust framework for analyzing protein hydrogen exchange.
    • The model highlights the importance of conformational flexibility and dynamics in protein behavior.
    • Further investigation into the physical significance of the unfolded state exchange distribution (kn) is warranted.

    Related Experiment Videos