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[Animal experimental studies of passive oral immunization against staphylococcal enterotoxin B (SEB)]
Abstract:
Intoxications by S. aureus enterotoxins are predominant in our country, together with enteric infections by Salmonella spp. For lack of a specific therapy the possibility was examined to neutralize orally provided toxin in vivo. For this purpose before and after the intoxication, respectively, antitoxin and normal rabbit serum as a control were also applied per os. In extensive trials SPF-mice were used as a model in spite of their insensitivity to staphylococcal toxins even with 10000 times the emetic dose for monkeys. By serological means, the rate of free toxin in stomach, intestinal contents and fecal matter after and before application of antiserum was identified as an indicator for the neutralization of the toxin in vivo. As toxin, S. aureus enterotoxin B (SEB) was used which could be identified in the samples up to three hours after oral feeding. Antiserum was obtained by immunization of rabbits with toxoid. The application of antiserum one hour after intoxication resulted in an average reduction of free toxin in the intestine of 87,5 and 86,5%, (10 independent trials) one and two hours, respectively after serum administration. In contrast, administration of antiserum one hour before experimental intoxication did not produce statistically confirmed differences between the experimental and the control animals.
Insights
Oral administration of antiserum effectively neutralizes Staphylococcus aureus enterotoxin B (SEB) in vivo when given after intoxication. This finding offers a potential therapeutic strategy for SEB-induced gastrointestinal issues.
Area of Science:
- Microbiology
- Immunology
- Toxicology
Context:
- Staphylococcus aureus enterotoxins are a significant cause of foodborne illness.
- Current therapeutic options for enterotoxin-induced intoxications are limited.
- In vivo neutralization of orally ingested toxins is an underexplored area.
Purpose:
- To investigate the efficacy of orally administered antiserum in neutralizing Staphylococcus aureus enterotoxin B (SEB) in vivo.
- To evaluate the impact of timing (pre- vs. post-intoxication) on the neutralizing effect of antiserum.
- To establish a method for quantifying toxin neutralization in gastrointestinal contents.
Summary:
- SPF-mice were used to model Staphylococcus aureus enterotoxin B (SEB) intoxication.
- Antiserum was administered orally either one hour before or one hour after SEB intoxication.
- Serological methods quantified free SEB in stomach, intestinal, and fecal contents to assess neutralization.
- Oral antiserum administration one hour post-intoxication significantly reduced free SEB in the intestine by over 86%.
Impact:
- Demonstrates the potential of oral antiserum administration as a therapeutic intervention for Staphylococcus aureus enterotoxin B intoxication.
- Provides evidence for the in vivo neutralization of orally ingested toxins.
- Highlights the importance of timely intervention for effective toxin neutralization.