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One-step purification of L-type pyruvate kinase by microimmunoaffinity chromatography
Abstract:
L-type pyruvate kinase from several tissues was purified by a micro-method of immunaffinity chromatography and the subunit structure analysed by SDS-electrophoresis (1-3). Whereas in adult human kidney L as well as L' seem to be present in the normal erythrocyte only L' subunits could be identified.