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Buffy coat-free red cell concentrates (RCC) from ACD, ACD-AG, and EDTA blood were prepared at the day of collection and resuspended with about one half of the packed red cell volume of a citrate-dextrose-sucrose-adenine-guanosine solution (CDS-AG). The posttransfusion survival of RBC in red cell suspensions (RCS) amounted to about 80% after 35 days of storage. 50 to 63% of the normal ATP level still remained. About 0.3% of red cells were hemolysed. 10% of red cells existed unchanged as discocytes, about 85% were transformed to echinocytes and 5% to spherocytes. The MCHC increased by about 25% due to a shrinkage of RBC. A much more hypertonic citrate-saline anticoagulant solution (420 mosmol/kg) increased the shrinkage, led to a higher hemolysis and lower posttransfusion survival rates.
Buffy coat-free red cell concentrates (RCC) from ACD, ACD-AG, and EDTA blood were prepared at the day of collection and resuspended with about one half of the packed red cell volume of a citrate-dextrose-sucrose-adenine-guanosine solution (CDS-AG). The posttransfusion survival of RBC in red cell suspensions (RCS) amounted to about 80% after 35 days of storage. 50 to 63% of the normal ATP level still remained. About 0.3% of red cells were hemolysed. 10% of red cells existed unchanged as discocytes, about 85% were transformed to echinocytes and 5% to spherocytes. The MCHC increased by about 25% due to a shrinkage of RBC. A much more hypertonic citrate-saline anticoagulant solution (420 mosmol/kg) increased the shrinkage, led to a higher hemolysis and lower posttransfusion survival rates.