[Primary structure of rhodopsin. I. Cyanogen bromide degradation peptides]

Carboxymethylated bovine rhodopsin was subjected to cyanogen bromide cleavage at methionine residues. The resultant products were fractionated into the two groups according to the solubility of peptides in 2 M guanidine hydrochloride. Gel-filtration on Bio-Gel P-30 in 80% formic acid of each group followed by rechromatography and high performance liquid chromatography resulted in 15 peptides embracing the whole polypeptide chain of rhodopsin. Amino acid sequence of these peptides was determined.