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Use of cholera toxoid in an enzyme-linked immunosorbent assay for antitoxin
Diagnostic Immunology
|January 1, 1983
Abstract:
A glutaraldehyde-inactivated toxoid was evaluated as a coating antigen in an ELISA for cholera antitoxin. A reference panel of 36 human sera with antitoxin levels determined by several other assay systems and 58 sera from an outbreak of illness due to Vibrio cholerae were studied. Toxoid compared favorably with two purified cholera toxins, and the microtiter assay using all three antigens was effective in detecting antibody in known convalescing cholera patients.