Golgi apparatus: distinct structure of acid phosphatase localization in regenerating human skeletal muscle fiber

The localization of acid phosphatase activity has been investigated ultrastructurally in regenerating human fetal skeletal muscle cells. The heavy reaction product was observed in the cisternae of Golgi apparatus and lysosomal vesicles, whereas the slight reaction product was also occasionally found in the nuclear envelope, endoplasmic reticulum and sarcoplasmic reticulum of the regenerating myocytes. The enzymatic basis for these deposits was confirmed by their absence in the incubation of the specimen in substrate-free medium and the enzyme inactivation of the specimen by exposure to NaF or heat.