Inulin single injection clearance. Microsample technique useful in children for determination of glomerular...

The agreement between single injection inulin (polyfructosan) clearance and two reference methods for glomerular filtration rate (GFR) determination, namely standard inulin constant infusion clearance and 51-Cr-EDTA single injection clearance, was investigated. Studies were also made to see whether different results were obtained when capillary and venous blood samples were used in the single injection clearance method. The investigation was made in children from one year of age, adult patients and volunteers. For the single injection technique with inulin or 51-Cr-EDTA, five to seven blood samples were taken in a period of up to 180 min after injection. A bi-exponential plasma disappearance curve was used for calculations of single injection clearances. Good agreement was obtained between venous inulin single injection clearance on the one hand, and standard inulin clearance (correlation coefficient r = 0.86) and 51-Cr-EDTA single injection clearance on the other (r = 0.85). With use of capillary blood samples in inulin single injection clearance, similar good agreement was obtained both with venous 51-Cr-EDTA and with venous inulin single injection clearance (r = 0.85 and r = 0.89 respectively). From these results we conclude that inulin single injection clearance is a reliable method for GFR determinations. This method is recommended, especially in children, as capillary blood samples can be used.