Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Free testosterone levels during danazol therapy.

B Nilsson, R Södergård, M G Damber

    Fertility and Sterility
    |April 1, 1983
    PubMed
    Summary

    Danazol treatment for endometriosis increases free testosterone (T) levels by displacing it from binding proteins. This rise in bioavailable T may explain many of danazol's clinical effects and side effects.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Expression of intestinal alkaline phosphatase in human organs.

    Histochemistry·1992
    Same author

    Ganglioside binding proteins of calf brain with ubiquitin-like N-terminals.

    European journal of biochemistry·1992
    Same author

    A randomized study of long-term out-patient treatment in alcoholics. Psychiatric treatment versus multimodal behavioural therapy, during 1 versus 2 years of treatment.

    Alcohol and alcoholism (Oxford, Oxfordshire)·1992
    Same author

    Detection and characterization of immunoconglutinins in patients with systemic lupus erythematosus (SLE): serial analysis in relation to disease course.

    Clinical and experimental immunology·1992
    Same author

    Prognostic significance of mucosal aneuploidy in stage Ta/T1 grade 3 carcinoma of the bladder.

    The Journal of urology·1992
    Same author

    A study of interface effects in 60Co beams using a thin-walled parallel plate ionization chamber.

    Medical physics·1992

    Area of Science:

    • Endocrinology
    • Pharmacology
    • Gynecology

    Background:

    • Danazol, a testosterone derivative, is clinically used for endometriosis.
    • Its mechanism of action is not fully understood, with androgenic side effects noted.
    • Danazol is known to displace testosterone (T) from sex-hormone-binding globulin (SHBG).

    Purpose of the Study:

    • To investigate the binding properties of danazol with SHBG and albumin.
    • To determine the impact of danazol therapy on T levels, including free and protein-bound fractions.
    • To explore the relationship between danazol's effects and alterations in free T levels.

    Main Methods:

    • Utilized labeled danazol in an aqueous two-phase equilibrium partition system to study binding.
    • Measured total testosterone (T), SHBG, and albumin levels in 16 women with endometriosis undergoing danazol treatment.
    • Calculated free and protein-bound T levels before and during danazol therapy.

    Main Results:

    • Danazol demonstrated binding affinity for SHBG and albumin.
    • A significant increase in free T levels was observed during danazol therapy compared to baseline.
    • No significant changes were noted in SHBG or albumin levels.

    Conclusions:

    • Danazol therapy leads to increased levels of free testosterone (T).
    • The displacement of T from binding proteins by danazol is a key factor.
    • Elevated free T likely contributes to both the therapeutic effects and androgenic side effects of danazol in endometriosis treatment.

    Related Experiment Videos