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Related Experiment Videos

Dye permeability at phase transitions in single and binary component phospholipid bilayers.

L F Braganza, B H Blott, T J Coe

    Biochimica Et Biophysica Acta
    |June 10, 1983
    PubMed
    Summary

    Phospholipid liposomes release encapsulated dyes at temperatures below their phase transitions, indicating bilayer permeability changes. Dye release is size-dependent and influenced by lipid composition and temperature.

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    Area of Science:

    • Biochemistry
    • Materials Science
    • Physical Chemistry

    Background:

    • Liposomes are widely used as drug delivery systems.
    • Understanding liposome permeability is crucial for drug delivery applications.
    • Phospholipid phase transitions significantly affect liposome membrane properties.

    Purpose of the Study:

    • To investigate the temperature-dependent permeability of DMPC, DPPC, and DMPC/DPPC mixture liposomes.
    • To determine the relationship between phospholipid phase transitions and dye release.
    • To assess the influence of encapsulated molecule size on liposome permeability.

    Main Methods:

    • Encapsulation of phenol red dye in multilamellar liposomes.
    • Preparation of liposomes from DMPC, DPPC, and their mixtures.

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  • Monitoring dye release as a function of temperature using optical absorbance.
  • Investigating the release of haemoglobin to assess size-dependent permeability.

    • Main Results:

    • Dye release initiated at pretransition temperatures for both DMPC and DPPC liposomes.
    • DPPC liposomes showed a permeability anomaly at their main phase transition (42°C).
    • Liposome permeability was size-dependent, with larger molecules like haemoglobin not being released.

    Conclusions:

    • Phospholipid bilayer permeability is significantly altered at pretransition and phase transition temperatures.
    • Liposome composition and temperature are critical factors controlling encapsulated dye release.
    • The study provides insights into liposome stability and drug delivery potential based on bilayer dynamics.