Monoclonal antibodies to a recombinant human leukocyte interferon (rIFN-alpha B)

Recombinant human leukocyte B interferon (rIFN-alpha B) was purified to approximately 60% on a column of monoclonal antibody to IFN-alpha. This partially purified rIFN-alpha B was in turn used as immunogen in obtaining four monoclonal antibodies to this recombinant interferon. All four antibodies, while showing varying degrees of cross-reactivity to some other rIFN-alpha subtypes, showed specificity to monomeric and dimeric forms of rIFN-alpha B only and not to the contaminants in the partially purified preparation as demonstrated in the Western blot immunoassay.