Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Accurate reference measurement for postmortem lung water.

M Julien, M R Flick, J M Hoeffel

    Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of Feeding Lactic Acid Bacteria and Fermented Milk on Specific and Nonspecific Immune Reponses of Mice Infected With Klebsiella pneumoniae AD-1.

    Journal of food protection·2019
    Same author

    Melanin-concentrating hormone in peripheral circulation in the human.

    The Journal of endocrinology·2017
    Same author

    Assessing Heterogeneity of Treatment Effect Using Real World Data.

    Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
    Same author

    Safety, tolerability, pharmacokinetics and pharmacodynamics of AZP-531, a first-in-class analogue of unacylated ghrelin, in healthy and overweight/obese subjects and subjects with type 2 diabetes.

    Diabetes, obesity & metabolism·2016
    Same author

    Elevated ratio of acylated to unacylated ghrelin in children and young adults with Prader-Willi syndrome.

    Endocrine·2015
    Same author

    The Industrial Revolution and the decline in death rates from tuberculosis.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2015
    Same journal

    Metabolic control of cardiac output response to exercise in McArdle's disease.

    Journal of applied physiology: respiratory, environmental and exercise physiology·1984
    Same journal

    Hypoxic insomnia: effects of carbon monoxide and acclimatization.

    Journal of applied physiology: respiratory, environmental and exercise physiology·1984
    Same journal

    Quiet-breathing vs. panting methods for determination of specific airway conductance.

    Journal of applied physiology: respiratory, environmental and exercise physiology·1984
    Same journal

    A new method for raising neonatal rabbits in a hypoxic environment.

    Journal of applied physiology: respiratory, environmental and exercise physiology·1984
    Same journal

    Modification of the cutaneous vascular response to exercise by local skin temperature.

    Journal of applied physiology: respiratory, environmental and exercise physiology·1984
    Same journal

    Temperature regulation during treadmill exercise in the rat.

    Journal of applied physiology: respiratory, environmental and exercise physiology·1984
    See all related articles

    Pulmonary edema increases dry lung weight, underestimating lung water accumulation. Hydroxyproline content offers a more accurate measure of lung water in edema quantification.

    Area of Science:

    • Pulmonary Medicine
    • Physiology
    • Biochemistry

    Background:

    • Pulmonary edema is a critical condition characterized by excess fluid in the lungs.
    • Accurate quantification of extravascular lung water is vital for assessing edema severity.
    • Traditional methods using dry lung weight may be confounded by changes in dry mass during edema.

    Purpose of the Study:

    • To evaluate the impact of increased dry blood-free lung weight on the ratio of extravascular lung water to dry lung weight in pulmonary edema.
    • To determine if hydroxyproline content provides a more accurate measure of lung water accumulation compared to dry lung weight.

    Main Methods:

    • Postmortem lung water, dry mass, and hydroxyproline content were measured in sheep with normal lungs, high-pressure edema, or increased permeability edema.

    Related Experiment Videos

  • Residual blood in the lungs was assessed using hemoglobin and 51Cr-tagged red blood cells.
  • The ratio of extravascular lung water to dry blood-free lung weight and to lung hydroxyproline content was calculated.

    • Main Results:

    • Extravascular lung water significantly increased in both high-pressure (64%) and increased permeability (82%) edema groups.
    • Dry blood-free lung weight was significantly elevated (33%) in the increased permeability edema group.
    • The ratio of extravascular lung water-to-dry blood-free lung weight underestimated lung water by approximately 50% in increased permeability edema.

    Conclusions:

    • Increased dry lung weight during pulmonary edema leads to underestimation of lung water when using the extravascular lung water-to-dry lung weight ratio.
    • Lung hydroxyproline content remains unaffected by edema and serves as a more accurate marker for quantifying extravascular lung water.
    • Hydroxyproline-based ratios offer improved accuracy in the assessment of pulmonary edema severity.