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Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
Published on: August 16, 2017
Use of statistical criteria for screening potential homologies in nucleic acid sequences
Abstract:
We proposed a simple formula to assess the statistical significance of homologous segments found in comparison of two nucleic acid sequences (Goad and Kanehisa, Nucleic Acids Res. 10, 247-263, 1982). This paper clarifies the basic assumptions of the formula and its reliability is examined by Monte Carlo calculations. The results were satisfactory for random sequences. The formula is a useful measure for screening potentially interesting homologies and it can be implemented in any search algorithms. Examples are given for the screening procedure in the graphic display version of the Goad-Kanehisa algorithm.
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