[Spectrophotometric analysis of amphotericin B in technological objects]

The same samples of the fermentation broth and mycelium of the amphotericin B-producing organism, (S. nodosus, strain 258) were assayed with the agar diffusion method and spectrophotometrically. The comparison of the results showed that the spectrophotometric findings did not depent on the wave lengths and the equation type. However, the values of the spectrophotometric assay were higher than those of the biological assay. This was partially due to the presence of a biologically inactive admixture of the heptaenic nature in the semiproducts of amphotericin B. At the same time when the strain, nutrient medium and biosynthetic conditions were stable, the results of the biological and spectrophotometric assays had a linear dependence. This allowed one to suggest equations for spectrophotometric determinations providing results correlating with those of the biological assay.