Luteinizing hormone secretion in ovariectomized gilts: effects of age, reproductive state and estrogen replacement

Crossbred gilts were ovariectomized (OVX) at 120, 150, 180 and 210 d of age to determine whether various characteristics of luteinizing hormone (LH) concentrations are influenced by age and reproductive state (prepuberal vs postpuberal). All 120-d-old gilts were prepuberal and all 210-d-old animals were postpuberal, whereas gilts 150 and 180 d old included both prepuberal and postpuberal animals. Blood was collected at 15-min intervals for 2 h, 2 d before OVX (d -2), and 2 (d +2), 8 (d +8) and 14 (d +14) d after OVX. Mean LH concentrations for prepuberal gilts were similar among age groups (P greater than .05) on d -2 and +2; however, LH increased (P greater than .05) from d -2 to +2. No change in LH secretion was found in postpuberal gilts during these two periods. After OVX, LH increased from d +2 to +14 in both prepuberal and postpuberal gilts in all age groups. In postpuberal gilts, LH increased linearly (P less than .05) between d +2 and +14; rate of increase accelerated with advancing age (P less than .01). In prepuberal gilts, LH increased in a nonlinear manner, but it did not increase between d +2 and +8. The increase observed in prepuberal and postpuberal gilts after OVX resulted primarily from an increase in magnitude of peak concentrations of LH. Implants of estradiol-17 beta (E2) were used to determine whether the postovariectomy increase in LH is affected differently by E2 in prepuberal and postpuberal gilts during advancing ages.(ABSTRACT TRUNCATED AT 250 WORDS)