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Related Experiment Videos

Fluorometric screening method for citrinin in corn, barley, and peanuts.

A L Trantham, D M Wilson

    Journal - Association of Official Analytical Chemists
    |January 1, 1984
    PubMed
    Summary

    This study presents a rapid fluorometric method for detecting citrinin, a mycotoxin, in grains and peanuts. The method offers efficient screening for citrinin contamination in agricultural commodities.

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    Area of Science:

    • Agricultural Science
    • Food Safety
    • Analytical Chemistry

    Background:

    • Citrinin is a mycotoxin produced by several species of *Penicillium* and *Aspergillus* fungi.
    • Contamination of food and feed with citrinin poses a significant risk to human and animal health.
    • Rapid and reliable detection methods are crucial for monitoring citrinin levels in agricultural products.

    Purpose of the Study:

    • To develop and validate a rapid fluorometric method for the detection of citrinin.
    • To assess the efficiency of the method in various agricultural commodities like corn, barley, and peanuts.
    • To provide a screening tool for citrinin contamination.

    Main Methods:

    • Extraction of citrinin using an acidified acetonitrile solution.
    • Partial purification of the extract via acid-base partition steps.
    • Quantification of citrinin using fluorometry.

    Main Results:

    • The fluorometric method demonstrated rapid screening capabilities for citrinin.
    • Mean recoveries of added citrinin ranged from 40.0% in peanuts to 66.6% in barley.
    • The method was effective for detecting citrinin at levels from 100-8000 ng/g.

    Conclusions:

    • The described fluorometric method is suitable for the rapid screening of citrinin in corn, barley, and peanuts.
    • The method provides a valuable tool for food safety assessments and quality control.
    • Further optimization may enhance recovery rates for improved accuracy.

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