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Protein efficiency ratio: AACC/ASTM collaborative study.

L R Hackler, C E Bodwell, M L Happich

    Journal - Association of Official Analytical Chemists
    |January 1, 1984
    PubMed
    Summary
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    This study standardized protein efficiency ratio (PER) bioassays across eight labs, finding adjusted PER (APER) reduced variation. Longer assay lengths (3-4 weeks) improved reproducibility over shorter ones.

    Area of Science:

    • Animal nutrition
    • Biochemical analysis
    • Food science

    Background:

    • Standardizing animal bioassays is crucial for reliable protein efficiency ratio (PER) determination.
    • Inter-laboratory variation in PER assays can impact nutritional assessments.
    • Methodological improvements are needed to enhance the consistency of PER data.

    Purpose of the Study:

    • To standardize methodology for animal bioassays determining protein efficiency ratios (PER).
    • To identify improvements that minimize inter-laboratory variation in PER studies.
    • To evaluate the impact of adaptation periods and assay lengths on PER results.

    Main Methods:

    • Eight laboratories conducted collaborative animal bioassays.
    • Protein efficiency ratios (PER) were determined over 1-, 2-, 3-, and 4-week periods with varying adaptation times.

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  • Six protein sources were tested, including casein, beef, lactalbumin, textured vegetable protein, peanut flour, and wheat flour.
  • Analyses included PER and adjusted PER (APER) calculations.

    • Main Results:

    • Significant effects on PER and APER were observed due to laboratories, adaptation length, and protein sources.
    • Adjusted PER (APER) values exhibited considerably less inter-laboratory variation compared to PER values.
    • Assay lengths of 3- and 4-weeks showed significantly lower reproducibility and repeatability variances than 2-week assays.
    • A significant interaction was found between laboratory, protein level, and assay length for PER values.

    Conclusions:

    • Adjusted PER (APER) offers improved standardization and reduced variability in protein bioassays.
    • Longer assay durations (3-4 weeks) enhance the reliability and reproducibility of protein efficiency ratio determination.
    • Standardized methodologies are essential for accurate comparative nutritional evaluations of protein sources.