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Updated: Sep 3, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
[Determination of serum iron; a comparison of two methods: atomic absorption and bathophenanthroline without
Unlabelled:
Two methods for the assay of serum iron are compared; determination by atomic absorption after deproteinisation, and spectrophotometric determination after liberation of the iron by a detergent, reduction with dithionite, and chelation with bathophenanthroline disulfonate, without deproteinisation.
Results:
Regression: y = 0.996 x --0.41 (s: atomic absorption; y: bathophenanthroline method; mumol/1 Fe; r = 0.986. Precision (CV) for a series of identical samples with 14.3 mumol/1: 2.0% for atomic absorption and 1.3% for bathophenanthroline.
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