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Related Experiment Videos

A rapid screening test for lactic aciduria.

M M Daalmans-De Lange, F A Hommes

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |June 1, 1978
    PubMed
    Summary

    A new rapid urine test accurately detects elevated lactate levels using an enzymatic reaction. This simple, inexpensive method is suitable for most laboratories, offering a reliable way to measure urine lactate.

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    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Diagnostic Assays

    Background:

    • Lactate is a key metabolic byproduct.
    • Elevated urine lactate levels can indicate certain physiological or pathological conditions.
    • Accurate and rapid measurement of urine lactate is clinically relevant.

    Purpose of the Study:

    • To describe a novel rapid test for determining lactate concentration in urine.
    • To validate the test's ability to differentiate between normal and elevated urine lactate levels.
    • To present a simple, inexpensive, and stable diagnostic tool for laboratory use.

    Main Methods:

    • The test utilizes the enzymatic decolourization of dichlorophenolindophenol by lactate.
    • The enzyme L-lactate cytochrome c oxidoreductase (EC 1.1.2.3) facilitates the reaction.
    • Vitamin C interference is mitigated through pre-treatment with charcoal.

    Main Results:

    • The described method adequately differentiates between normal and elevated urine lactate levels.
    • The test is characterized by its simplicity and low cost.
    • A stable and convenient compact testing unit can be assembled using a glass capillary.

    Conclusions:

    • A rapid, reliable, and cost-effective method for urine lactate determination has been developed.
    • The test is suitable for routine laboratory application.
    • This assay provides a valuable tool for assessing urine lactate levels.

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