mRNA sequences define an unusually restricted IgG response to oxazolone

The idiotypic analysis of hybridomas derived 7 and 14 days after primary immunization with oxazolone suggested that V-gene expression at these two stages was very different mRNA H and L sequences disclosed that day-7 antibody structures were highly conserved, which can be attributed to the existence of one VH and one VL gene. Conversely, two Ox-id- sequences differed considerably from Ox-id+ antibodies and were apparently unrelated to the previously defined genes.