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Analysis of the anti-azobenzenearsonate response at the molecular level
We have analysed, at the molecular level, the antibodies associated with the anti-azobenzenearsonate response of A/J and BALB/c mice as well as the genes that encode them. All antibodies expressing the predominant idiotype are derived by somatic mutation from a single VH gene. Antibody molecules express the idiotype regardless of their light chains or the JH segments which encode them. The D segment, however, does seem to be important for idiotype expression. The anti-azobenzenearsonate antibodies possessing the minor idiotype (36-60) are encoded by single, highly homologous VH genes in A/J and BALB/c mice. The nucleotide sequences of the VH genes from these two strains show only two nucleotide disparities. However, as opposed to A/J mice, the 36-60 idiotype in BALB/c appears to be the dominant idiotype among anti-azobenzenearsonate antibodies.
We have analysed, at the molecular level, the antibodies associated with the anti-azobenzenearsonate response of A/J and BALB/c mice as well as the genes that encode them. All antibodies expressing the predominant idiotype are derived by somatic mutation from a single VH gene. Antibody molecules express the idiotype regardless of their light chains or the JH segments which encode them. The D segment, however, does seem to be important for idiotype expression. The anti-azobenzenearsonate antibodies possessing the minor idiotype (36-60) are encoded by single, highly homologous VH genes in A/J and BALB/c mice. The nucleotide sequences of the VH genes from these two strains show only two nucleotide disparities. However, as opposed to A/J mice, the 36-60 idiotype in BALB/c appears to be the dominant idiotype among anti-azobenzenearsonate antibodies.