Oxygen-radical production during inflammation may be limited by oxygen concentration

The relationship between oxygen-radical production by rat polymorphonuclear leucocytes and O2 concentration was established by the measurement of luminol-dependent chemiluminescence at defined O2 concentrations. The O2 concentration that gave 50% of the maximum stimulated oxygen-radical production was 31 +/- 9 microM for non-opsonized latex beads and 22 +/- 9 microM for chemotactic peptide. The O2 concentration in rheumatoid synovial fluid was approx. 30 microM. It is therefore proposed that radical production at an inflammatory site may be limited by O2 concentration.