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Related Experiment Videos

Plasma contact activation and decrease of factor V activity on negatively-charged polyelectrolytes.

M C Boffa, B Dreyer, C Pusineri

    Thrombosis and Haemostasis
    |February 28, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Negatively-charged polymers activate plasma contact systems, causing kinin release and factor V reduction. This surface activation is transient, with polymers becoming inert after initial plasma contact.

    Area of Science:

    • Biomaterials Science
    • Hematology
    • Polymer Chemistry

    Background:

    • Artificial devices often utilize negatively-charged polymers.
    • Understanding polymer interactions with biological systems like plasma is crucial for device safety and efficacy.
    • Plasma contains complex clotting and kinin systems sensitive to surface interactions.

    Purpose of the Study:

    • To investigate the in vitro effects of negatively-charged polymers on plasma clotting and kinin systems.
    • To characterize the activation and subsequent inactivation of plasma components upon contact with polyelectrolyte complexes.
    • To determine the mechanisms behind factor V activity reduction in the presence of these polymers.

    Main Methods:

    • In vitro study using polyelectrolyte complexes and human plasma.

    Related Experiment Videos

  • Assay of contact activation, factor XII activity, prekallikrein activity, kinin release, and factor V activity.
  • Immunological detection of key plasma factors.
  • Incubation of plasma with polymers, followed by analysis and secondary incubation with fresh plasma.

    • Main Results:

    • Contact activation was observed as an immediate, transient, and surface-dependent phenomenon.
    • Plasma incubation with polymers led to decreased factor XII and prekallikrein activity, with significant kinin release.
    • Factor V activity decreased in a surface- and time-dependent manner, potentially due to adsorption onto sulfonated polymer groups.
    • Purified factor V showed immediate inactivation, suggesting direct adsorption, and further factor V loss occurred upon secondary plasma incubation.

    Conclusions:

    • Negatively-charged polymers can initiate contact activation of plasma, leading to kinin release and factor V inactivation.
    • The observed effects are transient, with the polymer surface becoming inert after initial contact.
    • Factor V inactivation appears to be a direct adsorption process related to the polymer's sulfonated groups, independent of contact factor activation.