Photometric oculometry. I. An analysis of the optical principles in slit-lamp fluorophotometry

The optical principles of slit-lamp fluorophotometry are analysed by aid of a mathematical model of the optical system. The analysis forms the theoretical basis for a slit-lamp technique called photometric oculometry, which makes possible an estimation of intraocular axial distances and axial length. The technique is based on a calculation--by aid of a mathematical model of the eye--of the ratio between intraocular movement of the slit-lamp focal plane and corresponding movement of the slit-lamp. Intraocular axial distances can be calculated by aid of this ratio and a direct measurement of the slit-lamp movement when the focal plane is moved in the optical axis from retina to cornea. These items are prerequisites for a quantitative determination of the blood-retinal barrier permeability to fluorescein during vitreous fluorophotometry.