Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A note on 'plotless' methods for estimating bacterial cell densities.

D Roser, D B Nedwell, A Gordon

The Journal of Applied Bacteriology

|April 1, 1984

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Stillbirth prevention: the challenges of public campaigns.

BJOG : an international journal of obstetrics and gynaecology·2017

Same author

Prevalence and predictors of early gestational weight gain associated with obesity risk in a diverse Australian antenatal population: a cross-sectional study.

BMC pregnancy and childbirth·2017

Same author

False-positive synovial fluid alpha-defensin test in a patient with acute gout affecting a prosthetic knee.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2017

Same author

Rural habitat as risk factor for hepatitis E virus seroconversion in HIV-infected patients: A prospective longitudinal study.

Zoonoses and public health·2017

Same author

EBRA-Digital release 2003 versus 1998: A comparison of instrument repeatability for measuring implant migration and wear.

Hip international : the journal of clinical and experimental research on hip pathology and therapy·2017

Same author

Differences in Gut Metabolites and Microbial Composition and Functions between Egyptian and U.S. Children Are Consistent with Their Diets.

mSystems·2017

Plotless methods offer a rapid way to estimate bacterial cell density, correlating well with traditional counts. This technique is efficient for various distributions and suitable for automated counting.

Area of Science:

Microbiology
Ecology
Biostatistics

Background:

Traditional bacterial cell counting relies on plot-related methods like direct microscopical counting.
Plotless techniques are established for higher plant populations but less explored for bacterial enumeration.

Purpose of the Study:

To compare plot and plotless methods for estimating bacterial cell densities.
To evaluate the efficiency and accuracy of a plotless method using distance measurements.

Main Methods:

Bacterial cell density estimation using distance measurements from a fixed point in a field of view.
Comparison of plotless method estimates with traditional total cell counts.
Application to bacterial samples from Hypholoma basidiocarps.

Related Experiment Videos

Main Results:

Plotless method estimates were rapidly obtained and directly correlated with total cell counts.
No significant differences were found between replicated counts using plotless and traditional methods.
The method accurately estimated densities across several orders of magnitude and various distribution patterns.

Conclusions:

Plotless techniques provide a rapid and comparable alternative to traditional methods for bacterial cell enumeration.
The distance-based plotless method is versatile, applicable to random and aggregated distributions, and suitable for automation.
This method shows promise for estimating bacterial numbers on diverse substrates and in automated counting systems.