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Net protein ratio data: AACC-ASTM collaborative study.

M L Happich, C E Bodwell, L R Hackler

    Journal - Association of Official Analytical Chemists
    |March 1, 1984
    PubMed
    Summary
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    Net protein ratio (NPR) studies showed that 14-day measurements reduced variability compared to 7-day data. Adjusting NPR to relative-NPR (R-NPR) decreased interlaboratory variability in protein source evaluation.

    Area of Science:

    • Animal Nutrition
    • Protein Quality Assessment
    • Comparative Nutrition

    Background:

    • Accurate assessment of protein quality is crucial for animal feed formulation and human nutrition.
    • The net protein ratio (NPR) is a common method for evaluating protein quality.
    • Interlaboratory variability can affect the reliability of protein quality studies.

    Purpose of the Study:

    • To evaluate the impact of measurement duration (7 vs. 14 days) and adaptation time on net protein ratio (NPR) variability.
    • To assess the effectiveness of relative-NPR (R-NPR) in reducing inter- and intralaboratory variability.
    • To compare the protein quality of various protein sources using NPR and R-NPR.

    Main Methods:

    • Seven laboratories participated in the study, evaluating six protein sources (casein, beef, lactalbumin, textured vegetable protein, peanut flour, wheat flour).

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  • Diets were formulated at 10% or 6% protein levels and fed to rats adapted for 0, 2, or 4 days.
  • Net protein ratio (NPR) was calculated using animal weight gain and feed consumption data at 7 or 14 days; R-NPR was calculated by adjusting NPR data against casein.
  • Analysis of variance was used to identify significant interactions and variability.

    • Main Results:

    • Significant interactions were found among laboratories, protein sources, and animal adaptation times (P < 0.05).
    • Using 14-day NPR values significantly decreased both inter- and intralaboratory variability compared to 7-day values.
    • Adjustment to R-NPR reduced interlaboratory variability but did not lower intralaboratory variability.
    • Increasing animal adaptation time did not consistently decrease variability or coefficients of variation.

    Conclusions:

    • Fourteen-day NPR measurements are superior to 7-day measurements for reducing variability in protein quality assessments.
    • Relative-NPR (R-NPR) adjustment effectively reduces interlaboratory variability, enhancing data comparability across different labs.
    • Standardizing measurement duration and considering R-NPR adjustments can improve the reliability of protein quality studies.