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The separation of bovine prothrombin and descarboxyprothrombin by high-performance liquid chromatography
Analytical Biochemistry
|February 1, 1984
Abstract:
Prothrombin contains 10 gamma-carboxyglutamic acid (Gla) residues which are absent in the warfarin-induced descarboxyprothrombin; hence prothrombin has 10 more negative groups than has descarboxyprothrombin. The two proteins can be separated by HPLC with the aid of an anion-exchange column. Plasma from warfarin-treated animals could be analyzed without pretreatment of the samples and a full analysis was obtained in 30 min.