Properties of [3H]diazepam binding sites on cultured murine glia and neurons

[3H]Diazepam binding was assayed in situ on living cultures of fetal mouse cerebral cortex or glia in an attempt to further characterize the high and low affinity binding sites. Mixed neuronal-glial cultures were found to have a high (Kd approximately equal to 10 nM) as well as a low (Kd approximately equal to 240 nM) affinity binding site. Glial cultures also had a similarly high affinity site (Kd 13 nM). In both types of cultures, the high affinity site was Ro 5-4864 sensitive and clonazepam resistant. Since Ro 5-4864 has particular affinity for non-neuronal elements and clonazepam for neuronal elements, the data suggest that the high affinity binding site may be localized to glial elements and the low affinity site primarily neuronal.