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"Cytomic" data system modules--modern electronic devices for flow cytometric data handling and presentation.

V Kachel, K Schedler, H Schneider

    Cytometry
    |May 1, 1984
    PubMed
    Summary

    The Cytomic modular data system offers flexible flow cytometry solutions, adapting to single-parameter analyses or complex multi-parameter cell sorting needs. This adaptable system enhances experimental efficiency for diverse research requirements.

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    Area of Science:

    • Biotechnology
    • Cell Biology
    • Analytical Chemistry

    Background:

    • Flow cytometry is a powerful technique for analyzing cell characteristics.
    • Complex flow cytometric investigations require sophisticated data acquisition and sorting capabilities.
    • Simpler analyses can often be addressed with single-parameter measurements.

    Purpose of the Study:

    • To introduce the Cytomic modular data system for adaptable flow cytometry.
    • To present a system capable of handling both simple and complex flow cytometry tasks.
    • To describe a modular approach for scalable flow cytometry data analysis and cell sorting.

    Main Methods:

    • The Cytomic modular data system comprises four distinct modules: CYTOMIC 12 (1-2 parameters), CYTOMIC 123 (3 parameters), CYTOMIC SORT (cell sorting), and CYTOMIC LIST (list mode data acquisition).

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  • Each module features a Z80 microprocessor, dedicated peripherals (keyboard, display, power supply), and an intermodule communication bus.
  • Modules can function independently or be integrated into a larger system, allowing for progressive expansion.

    • Main Results:

    • The modular design allows users to start with basic functionality (e.g., one- and two-parameter analysis) and expand as needed.
    • The system supports various flow cytometry applications, from basic analysis to advanced cell sorting with up to eight parameters.
    • Each module is pre-programmed with functions optimized for flow cytometry, ensuring ease of use and efficiency.

    Conclusions:

    • The Cytomic modular data system provides a flexible and scalable solution for diverse flow cytometry needs.
    • Its modular architecture allows for cost-effective implementation and future upgrades.
    • This system enhances experimental throughput and adaptability in cell analysis and sorting applications.