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Updated: Aug 11, 2026

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
Published on: July 9, 2019
Discovery and initial characterization of a new conditional (temperature-sensitive) maternal effect mutation in the
Abstract:
The discovery of a new temperature-sensitive maternal effect mutation in Ambystoma mexicanum is described. The new gene (ts-1) was recognized when 100% of the eggs spawned by homozygous females failed to develop past early gastrulation when reared at 25 degrees C. Eggs raised at 10 degrees C developed normally to sexual maturity. A temperature-sensitive period during blastulation was identified by a preliminary series of temperature shifts. Histologic examination revealed that nuclear abnormalities, especially chromosomal bridges, characterized ts-1 embryos reared beyond the ninth cleavage at the restrictive temperature (25 degrees C).

