Study of binding of warfarin to serum albumins by high-performance liquid chromatography

The binding of warfarin to human serum albumin and bovine serum albumin, respectively, was studied by high-performance liquid chromatography (HPLC). Based upon the Hummel - Dreyer method, two techniques were developed: the internal calibration and the external calibration. The results obtained by the HPLC method and those obtained by the classical method (equilibrium dialysis) were compared. The external calibration method seems to be superior to others for its simplicity, speed and convenience.