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Related Experiment Videos

Placenta as a selective barrier to cellular traffic.

R D Hunziker, P Gambel, T G Wegmann

    Journal of Immunology (Baltimore, Md. : 1950)
    |August 1, 1984
    PubMed
    Summary

    Maternal cell trafficking across the placenta is rare in mice. While some red blood cells (RBC) cross, significant numbers of white blood cells (WBC) typically do not enter the fetus.

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    Lack of class I major histocompatibility antigens on trophoblast of periimplantation blastocysts and term placenta in the pig.

    Biology of reproduction·1999

    Area of Science:

    • Reproductive biology
    • Immunology
    • Developmental biology

    Background:

    • Understanding maternal-fetal cell exchange is crucial for pregnancy outcomes.
    • Previous studies have yielded conflicting results regarding transplacental cell passage.

    Purpose of the Study:

    • To quantify maternal cell trafficking into the fetus during murine pregnancy.
    • To determine the extent of red blood cell (RBC) and white blood cell (WBC) transfer.

    Main Methods:

    • Utilized genetic markers (glucose phosphate isomerase, GPI) to track maternal cells in offspring.
    • Employed fluorescein tagging of maternal RBC and WBC, followed by intravenous injection into pregnant mice.
    • Analyzed fetal tissues (liver, blood, spleen) using fluorescence-activated cell sorting (FACS) at various gestational stages.

    Main Results:

    • Only two out of 172 offspring showed evidence of maternal cell trafficking using GPI.
    • Fluorescein-labeled RBC were detected in neonates, but maternal WBC were found in minimal numbers (<225 cells).
    • The sensitive assay indicated that significant maternal WBC transfer is uncommon.

    Conclusions:

    • Transplacental passage of significant numbers of maternal white blood cells into the fetus is rare.
    • The limited maternal cell transfer observed may be associated with placental abnormalities.

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