Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement factor adsorption on solid surfaces--an ellipsometric method for investigation of quantitative aspects.

H Elwing, C Dahlgren, R Harrison

    Journal of Immunological Methods
    |July 6, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Use of hydrogen-sensitive Pd-MOS materials in biochemical analysis.

    Applied biochemistry and biotechnology·2013
    Same author

    Gradients in surface nanotopography used to study platelet adhesion and activation.

    Colloids and surfaces. B, Biointerfaces·2013
    Same author

    What formyl peptide receptors, if any, are triggered by compound 43 and lipoxin A4?

    Scandinavian journal of immunology·2011
    Same author

    Quasi three-dimensional, n-bit optical memory based on the ellipsometric principle: model calculations.

    Applied optics·2010
    Same author

    Lipoxin A(4) metabolites/analogues from two commercial sources have no effects on TNF-alpha-mediated priming or activation through the neutrophil formyl peptide receptors.

    Scandinavian journal of immunology·2009
    Same author

    Change of colloidal and surface properties of Mytilus edulis foot protein 1 in the presence of an oxidation (NaIO4) or a complex-binding (Cu2+) agent.

    Biomacromolecules·2009
    Same journal

    Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

    Journal of immunological methods·2026
    Same journal

    Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

    Journal of immunological methods·2026
    Same journal

    Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

    Journal of immunological methods·2026
    Same journal

    Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

    Journal of immunological methods·2026
    Same journal

    Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

    Journal of immunological methods·2026
    Same journal

    Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

    Journal of immunological methods·2026
    See all related articles

    Ellipsometry quantifies adsorbed proteins on surfaces. This study shows IgG adsorption impacts complement C3 binding and human polymorphonuclear leucocyte (PMNL) migration, revealing key interactions.

    Area of Science:

    • Biophysical Chemistry
    • Immunology
    • Surface Science

    Background:

    • Quantifying biomolecular interactions on surfaces is crucial for understanding biological processes.
    • Antibody-mediated immune responses involve complex interactions between proteins and cells.

    Purpose of the Study:

    • To quantify organic material adsorption from human sera onto antibody-coated surfaces using ellipsometry.
    • To investigate the relationship between immunoglobulin G (IgG) adsorption levels and complement factor binding (C3).
    • To measure the effect of IgG adsorption on human polymorphonuclear leucocyte (PMNL) migration inhibition.

    Main Methods:

    • Ellipsometry was employed to measure the adsorption of organic material from human sera onto antibody-coated surfaces.
    • Complement component C3 deposition was assessed on surfaces with varying IgG adsorption.

    Related Experiment Videos

  • A modified ellipsometry method quantified the inhibition of PMNL locomotion.

    • Main Results:

    • Maximal adsorption of organic material occurred at approximately 0.5 µg/cm² of IgG.
    • Complement component C3 was detected on antibody surfaces, but not C5, C8, or C9.
    • PMNL migration inhibition was observed within a narrow IgG adsorption range of 0.2 to 0.5 µg/cm².

    Conclusions:

    • IgG adsorbed on surfaces may lack binding sites for complement factors beyond C3.
    • The study highlights quantitative relationships between antibody-dependent complement activation and cellular responses like PMNL migration.
    • Ellipsometry provides a valuable tool for studying these complex immunological interactions.