Learned tolerance to ethanol in the spinal cord

Learning has been claimed to be of major importance in the development of tolerance to ethanol. In the present study we investigated the influence of learning on tolerance to ethanol-induced inhibition of a spinal reflex (tail-flick response) in intact and spinal rats. On day 1 and 9, groups of rats were injected with either ethanol 2.5 g/kg IP or saline 30 min prior to tail-flick testing. On days 2-8 the groups were treated differently in order to reveal the importance of the drug alone, the test alone and the combination of the two on development of tolerance. On day 10, the rats rendered tolerant in the home room were transferred to a new test room to be tested. Both in intact and spinal rats development of tolerance was observed only if the animals were repetitively tested while intoxicated. Tolerance acquired in the home room was not attenuated by transfer to a new environment. Results in the spinal rats suggested that adaptive mechanisms leading to tolerance may also be located in the spinal cord. The tolerance observed may be regarded as learned from practice while intoxicated.