A short-term screening test for teratogens using differentiating neuroblastoma cells in vitro

Thirty-nine teratogenic and 18 nonteratogenic compounds were tested using an assay based on the ability of agents to interfere with normal growth and differentiation of murine neuroblastoma cells (clone N1E-115) in culture. Induction of differentiation in cells under growth-promoting conditions and inhibition of differentiating cells over a period of 7 days was dose-dependent, with the lowest effective dose not being highly toxic. Eighty-six percent of the compounds were correctly identified by the assay. The proportions of both false negatives (10%) and false positives (22%) were of the same order or better than in current, comparable tests. The possibilities offered by the system in rapid screening for teratogenic potential of environmental agents are discussed.