Related Experiment Videos
Leucocyte depleted blood
Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|January 1, 1984
Summary
Non-haemolytic febrile transfusion reactions (NHFTR) can be prevented by transfusing leukocyte-depleted red cells. Various methods exist, with filtration and sedimentation offering effective leukocyte depletion and minimal red cell loss.
Area of Science:
- Transfusion Medicine
- Immunology
Background:
- Non-haemolytic febrile transfusion reactions (NHFTR) are frequently linked to leukocyte antibodies, particularly in patients receiving multiple transfusions.
- These reactions pose a clinical challenge, necessitating strategies to mitigate their occurrence.
Purpose of the Study:
- To review and compare various methods for leukocyte depletion of red blood cells.
- To evaluate the efficacy and limitations of different techniques in preventing NHFTR.
Main Methods:
- Comparison of manual centrifugation, saline washing, sedimentation with polymers (dextran), filtration, microaggregate filtration, and freezing/thawing.
- Assessment of leukocyte depletion efficiency and red cell loss for each method.
Main Results:
- Manual centrifugation is least effective; saline washing can be tedious or cause red cell loss.
- Sedimentation and filtration offer excellent leukocyte depletion with minimal red cell loss.
- Microaggregate filtration is a cost-effective bedside option; freezing/thawing is effective but costly.
Conclusions:
- Leukocyte depletion of at least 80% is recommended for preventing NHFTR.
- The optimal method depends on available resources, cost, clinical requirements (e.g., platelet depletion), and institutional experience.