[Correlations between immunoenzymatic test, neutralization test and fluorescent focus reduction test in rabies...

Three methods have been used and compared for the detection and titration of antibodies in the serum of humans receiving post-exposure treatment with tissue culture inactivated rabies vaccines: mouse seroneutralization test, fluorescent focus reduction test and immunoenzymatic test. Results have shown that the fluorescent focus reduction test has a sensitivity and specificity which are equivalent to the mouse seroneutralization test with the advantage of giving results in 30 hr instead of 21 days, and that the immunoenzymatic test which avoids the use of laboratory animals and/or cell cultures, and which gives results in 5 hr, may advantageously replace the neutralization methods, particularly for the testing of large numbers of sera.